Cultivar identi®cation of `Yuzu' (Citrus junos Sieb. ex Tanaka) and related acid citrus by leaf isozymes
نویسندگان
چکیده
Leaf extracts of 27 `Yuzu' and related acid citrus cultivars were analyzed using polyacrylamide gel electrophoresis for isozyme variation of glutamate oxaloacetate transaminase (GOT) and shikimate dehydrogenase (SDH). SDH yielded 12 different isozyme phenotypes and six cultivars were discriminated by this enzyme alone. GOT produced 10 different isozyme phenotypes and four cultivars were separated. When both enzyme systems were taken together, 16 cultivars (59%) were uniquely discriminated and the rest could be classi®ed into four groups of 2±4 cultivars each. Mutation originated cultivars could not be discriminated. Differences between cultivars suggested that isozymes may provide useful markers for cultivar identi®cation. # 2001 Elsevier Science B.V. All rights reserved.
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